2020 · 126 citations · 0 references
Life SciencesBiotechnologyMolecular BiologyGenomicsMolecular VirologyVirologyDna LigaseDna SequencingNext-generation SequencingNext-generation Sequencing In OncologyNanopore SequencingProtocol ApplicabilityProtocol AssistanceSars-cov-2 Infection
Amplicon sequencing protocol for SARS-CoV-2 v3 (LoCost) We thank the ARTIC network, Oxford Nanopore Technologies, New England Biolabs, BCCDC, COG-UK, CanCOGen and protocols.io commenters for their assistance developing this protocol. Changes in this version: -Up to 95 samples per run with EXP-NBD196 native barcode kit -Substitution of SuperScript IV for LunaScript RT SuperMix and reaction volume reduced to 10 uL. -Substitution of Ultra II Ligation Module for Blunt/TA Ligase Master Mix and reaction volume reduced to 10 µL. -Native barcode ligation reaction volume reduced to 10 uL. -SFB wash volume reduced.