International Journal of Electrochemical Science · 2012 · 24 citations · 20 references
EngineeringBiotechnologyChemical EngineeringBiosensorsElectroanalytical MethodsElectrochemistryElectrochemical BiosensorsElectrochemical DetectionElectrochemical SensorsElectrode MaterialsEnzymatic BiosensorsEnzyme-based BiosensorsAmperometric BiosensorsAmperometric DetectionAmperometric SensingCarbon Paste ElectrodesGlucose BiosensorsGlucose OxidaseGlucose SensorsLinear Range
In this study, a novel carbon paste electrode using the salt form of polyaniline (pani)-activated carbon composite sensitive to glucose, was prepared. Glucose oxidase enzyme was immobilized to modified carbon paste electrode by cross-linking with glutaraldehyde. Determination of glucose was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V vs. Ag/AgCl. The effects of pH and temperature were investigated and optimum parameters were found to be 5.0 and 65 °C, respectively. The linear working range of the electrode was 5.0×10-7 - 1.0×10-5 M, R2 =0.980. The storage stability and operation stability of the enzyme electrode were also studied.
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