Low-Potential Amperometric Determination of Reduced Glutathione at Physiological pH with an Electrodeposited Cu Nanoparticles Modified Pt Electrode

Abstract

The electrodeposition of Cu nanoparticles was investigated at Pt electrode in 10 mM Cu(NO3)2 in the presence and absence of NH4Cl. Pure Cu nanoparticles was synthesized in a solution containing NH4Cl, and Cu/Cu2O composite film was obtained without NH4Cl. In addition, rare Cu decanedrons was electrochemically synthesized in 10 mM Cu(NO3)2+0.1 M NaNO3. The electrodeposited Cu nanoparticles exhibits excellent electrocatalytic activity toward oxidation of reduced glutathione (GSH) at a super low potential and the electrocatalytic mechanism was discussed, which was first used for the electrochemical determination of GSH at physiological pH. Furthermore, the common interfering species, including ascorbic acid (AA), dopamine (DA), uric acid (UA), and glucose, were examined for the electrochemical detection of GSH, and the results showed that the oxidation from the interfering compounds was effectively avoided because of the super low detection potential.