Adhesion to laminin-1 and collagen IV induces the formation of Ca ²⁺ microdomains that sensitize mouse T cells for activation

Abstract

During an immune response, T cells migrate from blood vessel walls into inflamed tissues by migrating across the endothelium and through extracellular matrix (ECM). Integrins facilitate T cell binding to endothelial cells and ECM proteins. Here, we report that Ca²⁺ microdomains observed in the absence of T cell receptor (TCR)/CD3 stimulation are initial signaling events triggered by adhesion to ECM proteins that increase the sensitivity of primary murine T cells to activation. Adhesion to the ECM proteins collagen IV and laminin-1 increased the number of Ca²⁺ microdomains in a manner dependent on the kinase FAK, phospholipase C (PLC), and all three inositol 1,4,5-trisphosphate receptor (IP₃R) subtypes and promoted the nuclear translocation of the transcription factor NFAT-1. Mathematical modeling predicted that the formation of adhesion-dependent Ca²⁺ microdomains required the concerted activity of two to six IP₃Rs and ORAI1 channels to achieve the increase in the Ca²⁺ concentration in the ER-plasma membrane junction that was observed experimentally and that required SOCE. Further, adhesion-dependent Ca²⁺ microdomains were important for the magnitude of the TCR-induced activation of T cells on collagen IV as assessed by the global Ca²⁺ response and NFAT-1 nuclear translocation. Thus, adhesion to collagen IV and laminin-1 sensitizes T cells through a mechanism involving the formation of Ca²⁺ microdomains, and blocking this low-level sensitization decreases T cell activation upon TCR engagement.