NIR-II Responsive Nanohybrids Incorporating Thermosensitive Hydrogel as Sprayable Dressing for Multidrug-Resistant-Bacteria Infected Wound Management

Abstract

Developing an effective dressing against bacterial infection and synchronously addressing wound complications, such as bleeding, long-term inflammation, and reinfection, are highly desirable in clinical practice. In this work, a second near-infrared (NIR-II) responsive nanohybrid consisting of <b>i</b>mipenem encapsulated <b>l</b>iposome with <b>g</b>old-shell and lipopolysaccharide (LPS)-targeting <b>a</b>ptamer, namely <b>ILGA</b>, is constructed for bacteria elimination. Benefiting from the delicate structure, <b>ILGA</b> exhibits strong affinity and a reliable photothermal/antibiotic therapeutic effect toward multidrug-resistant <i>Pseudomonas aeruginosa</i> (<i>MDR-PA</i>). Furthermore, by incorporating <b>ILGA</b> with a thermosensitive hydrogel poly(lactic-<i>co</i>-glycolic acid)-polyethylene glycol-poly(lactic-<i>co</i>-glycolic acid) (PLGA-PEG-PLGA), a sprayable dressing <b>ILGA@Gel</b> was prepared, which enables a quick on-demand gelation (10 s) for wound hemostasis and offers excellent photothermal/antibiotic efficacy to sterilize the infected wound. Additionally, <b>ILGA@Gel</b> provides satisfactory wound-healing environments by reeducating wound-associated macrophages for inflammation alleviation and forming a gel layer to block exogenous bacterial reinfection. This biomimetic hydrogel reveals excellent bacteria eradication and wound recovery effectiveness, demonstrating its promising potential for managing complicated infected wounds.