Composite Hemostat Spray Seals Post-Surgical Blood Burst and Ameliorates Bacteria-Arised Inflammation for Expediting Wound Healing

Abstract

Postoperative bleeding and infection are the main causes of death. Although a commercial zeolite-represented hemostatic agent has been widely used, zeolite-induced thermal damages to tissues are unresolved, and antibacterial and wound healing are desirably demanded but challenging. Herein, a composite zeolite-based hemostat spray consisting of Ag-doped β zeolite (Ag-βZ) and coupled fibrinogen/thrombin (F/T) has been developed to seal postsurgical blood burst. Systematic experiments reveal that the F/T can be switched into fibrin gel as the physical barrier and cover Ag-βZ, which imparts the composite hemostats with the most robust hemostasis ability associated with the lowest bleeding amount and the shortest hemostasis time. Concurrently, the physical barrier can shield Ag-βZ from touching water, reduce solvothermal release, and significantly minimize temperature, consequently resulting in no obvious thermal effect on the surrounding tissues. Importantly, the in situ doping of Ag can produce reactive oxygen species to kill bacteria and ameliorate bacteria-aroused inflammation, which can be also leveraged to promote cell expansion for expediting wound healing. The clinically approved components guarantee biosafety, paving a solid foundation for clinical translation.