Isolation of Hv-CRKP with co-production of three carbapenemases (blaKPC, blaOXA-181 or OXA-232, and blaNDM-1) and a virulence plasmid: a study from a Chinese tertiary hospital

Abstract

In our investigation, we have observed the emergence of hv-CRKP carrying <i>bla</i>_OXA-48-like genes, which identified two genetic relationships: clonal transmission and plasmid transmission. PBRT analysis showed that these genes were mainly carried on ColE-type, IncF, and IncX3 plasmids. These isolates have been shown to be hypervirulent <i>in vitro</i> and <i>in vivo</i>. Additionally, eight clinical isolates of hv-CRKP were identified as carrying three carbapenem-resistant genes (<i>bla</i>_KPC, <i>bla</i>_{OXA-181 or OXA-232}, and <i>bla</i>_NDM-1) and carrying a pLVPK-like virulent plasmid. Hence, our findings highlight the need for further investigation and active surveillance of hypervirulent OXA-48-like producing Hv-CRKP isolates to control their transmission.