Development of a two-photon fluorescent probe for imaging hydrogen sulfide (H₂S) in living cells and zebrafish

Abstract

We present a new two-photon fluorescent probe (T-HS) for the detection of H₂S. With the addition of hydrogen sulfide, the absorption and fluorescence spectra of the probe show regular changes. The probe exhibited favorable properties, such as large turn-on fluorescence signal, good selectivity and low cytotoxicity. Moreover, the probe T-HS was successfully used for the fluorescence imaging of H₂S in live cells and zebrafish.