Effects of the Vitamin D3 on Alleviating the Oxidative Stress Induced by Diquat in Wenchang Chickens

Abstract

Vitamin D₃ (VD₃) is an indispensable micronutrient in livestock and poultry feed. Its function in antioxidant stress has been reported. We investigate whether the addition of different concentrations of VD₃ to the diet affects the production performance, slaughter performance, meat quality, organ index, and gut injury on the diquat (DQ)-induced model of oxidative stress in Wenchang chickens. Four hundred and eighty one-day-old chickens were randomly divided into six groups: control (basal diet), 4000 VD (basal diet + VD₃ 4000 IU per kg feed intake), 1000 VD+DI (DQ, basal diet + VD₃ 1000 IU per kg feed intake), 2000 VD+DI (DQ, basal diet + VD₃ 2000 IU per kg feed intake), and 4000 VD+DI (DQ, basal diet + VD₃ 4000 IU per kg feed intake). The results showed that the addition of VD₃ to the diet promoted DQ-induced weight loss and reduced ADFI, slaughter rate, splenic index, and pH after 1 h and 24 h in the leg muscles. VD₃ decreased the increase in content of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) among proinflammatory cytokines (<i>p <</i> 0.05) and increased the reduction in anti-inflammatory cytokines content of interleukin-10 (IL-10) (<i>p <</i> 0.05) induced by DQ. In addition, liver and kidney injury biomarkers and the intestinal permeability index in serum were disordered after treatment with DQ (<i>p <</i> 0.05). VD₃ perfected the increase of D-lactic acid (D-LA), diamine oxidase (DAO), total cholesterol (T-CHO), creatinine (CR), blood urea nitrogen (BUN), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) content, aspartate transaminase (AST), alanine transaminase (ALT), and lactate dehydrogenase (LDH) activity (<i>p <</i> 0.05); it increased the decrease of albumin (ALB) content (<i>p <</i> 0.05). Meanwhile, VD₃ regulated the intestinal morphology and intestinal barrier. Moreover, DQ induced a decrease in total antioxidant capacity and antioxidant enzyme activity in the serum, liver, and jejunum (<i>p <</i> 0.05), and an increase in malonaldehyde (MDA) content (<i>p <</i> 0.05). However, the addition of different levels of VD₃ could alleviate the above phenomenon of oxidative stress in Wenchang chickens to different degrees. Thus, this research suggested that the addition of VD₃ can relieve the DQ-induced oxidative stress of Wenchang chickens, and the level of VD₃ acquisition is positively correlated with the remission effect.