Anthropogenic impacts on the nitrate pollution in an urban river: Insights from a combination of natural-abundance and paired isotopes

Abstract

Urban rivers are often characterized by high nitrate (NO₃^-) loadings. High NO₃^- loadings cause water quality and ecological damages, which undermines the sustainable development of cities. To date, the drivers of these high NO₃^- loadings remain unclear. This study, for the first time, integrated natural-abundance isotopes (δ¹⁵ N/δ¹⁸O-NO₃^- and δD/δ¹⁸O-H₂O) and ¹⁵N-pairing techniques to comprehensively reveal the anthropogenic impacts on the NO₃^- pollution in an urban river. Natural-abundance isotopes suggested that in both the wet and dry seasons, the NO₃^- was predominantly from the conservative mixing of different sources, and biological NO₃^- removal was minor. The ¹⁵N-pairing experiments supported the natural-abundance isotope data, quantitatively showing that in-soil nitrification was prevailing, while NO₃^- removal processes (denitrification, anammox, and dissimilatory NO₃^- reduction to ammonium) were weak. A Bayesian isotope-mixing model showed that soil sources (soil organic nitrogen and chemical fertilizer) dominated the NO₃^- in the upper reaches, while in the lower reaches, the impermeable riparian zone short-circuited the access of soils to the river. Here, the wastewater treatment plants became a significant source of NO₃^-. This study quantitatively revealed the drivers of high NO₃^- loadings in an urban river, and generated important clues for effective NO₃^- pollution control and remediation in urban rivers.