Biological approach to synthesize TiO2 nanoparticles using Staphylococcus aureus for antibacterial and antibiofilm applications

Abstract

Nano-sized materials have been an important tool in basic and applied sciences. A novel, low cost, green and reproducible bacteria, Staphylococcus aureus mediated biosynthesis of titanium dioxide nanoparticles (TiO2 NPs) was reported in the present study. Initial conformational studies were done using UV-visible spectroscopy and confirmed the synthesis of TiO2 NPs in the broth. The detailed characterization of the TiO2NPs was carried out using SEM, XRD, FTIR and Raman spectroscopy. From the SEM, it was confirmed that the sample showed the NPs were smooth and spherical with an average diameter of about 20nm. From FTIR analysis, it was confirmed that the TiO2 nanoparticles are crystalline in nature, which was confirmed by the FTIR peak at 518cm-1 corresponds to the TiO2 vibration present in a crystalline structure. Additionally, the synthesized NPs were also characterized by Transmission Electron Microscopy (TEM) and Particle size analyzer. This study was aimed to determine the antibacterial and antibiofilm activity of Titanium oxide nanoparticles against both Gram-positive and Gram-negative bacterial species and significant positive results against Bacillus subtilis and Escherichia coli were observed.