Protective Effect of Ganoderma lucidum Spore Powder on Acute Liver Injury in Mice and its Regulation of Gut Microbiota

Abstract

Compared with those in the 50% ethanol model group (MG), sporoderm-broken GLSP significantly reduced serum AST and ALT levels (<i>p <</i> 0.0001) and the release of the inflammatory factors, including IL-1β, IL-18, and TNF-α (<i>p <</i> 0.0001), and effectively improved the pathological state of liver cells; sporoderm-unbroken GLSP significantly reduced the ALT content (<i>p</i> = 0.0002) and the release of the inflammatory factors, including IL-1β (<i>p <</i> 0.0001), IL-18 (<i>p</i> = 0.0018), and TNF-α (<i>p</i> = 0.0005), and reduced the serum AST content, but the reduction was not significant; compared with the gut microbiota of the MG, sporoderm-broken GLSP reduced the levels of <i>Verrucomicrobia</i> and <i>Escherichia_Shigella</i>, increased the relative abundance of beneficial bacteria such as <i>Bacteroidetes</i>, and decreased the abundance levels of harmful bacteria, such as <i>Proteobacteria</i> and <i>Candidatus</i>_<i>Saccharibacteria</i>; sporoderm-unbroken GLSP could reduce the abundance levels of harmful bacteria, such as <i>Verrucomicrobia</i> and <i>Candidatus_Saccharibacteria</i>; and GLSP treatment alleviates the downregulation of the levels of translation, ribosome structure and biogenesis, and lipid transport and metabolism in liver-injured mice; Conclusions: GLSP can alleviate the imbalance of gut microbiota and improve liver injury, and the effect of sporoderm-broken GLSP is better.