A Near-Infrared Fluorescent Probe for Recognition of Hypochlorite Anions Based on Dicyanoisophorone Skeleton

Abstract

A novel near-infrared (NIR) fluorescent probe (<b>SWJT-9</b>) was designed and synthesized for the detection of hypochlorite anion (ClO^-) using a diaminomaleonitrile group as the recognition site. <b>SWJT-9</b> had large Stokes shift (237 nm) and showed an excellent NIR fluorescence response to ClO^- with the color change under the visible light. It showed a low detection limit (24.7 nM), high selectivity, and rapid detection (within 2 min) for ClO^-. The new detection mechanism of <b>SWJT-9</b> on ClO^- was confirmed by ¹H NMR, MS spectrum, and the density functional theory (DFT) calculations. In addition, the probe was successfully used to detect ClO^- in HeLa cells.