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<i>In Vitro</i> Immune Response of Human Peripheral Lymphocytes
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1977
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Adaptive Immune SystemPwm-stimulated PblT-regulatory CellImmunologyImmune RegulationImmunologic MechanismInnate ImmunityImmune SystemImmunotherapyActive SubstanceIntrinsic ImmunityAutoimmune DiseaseAutoimmunityT Cell ImmunityHuman Peripheral LymphocytesCell BiologyImmune Cell DevelopmentMhc BarrierMedicine
Abstract Human peripheral lymphocytes (PBL) upon stimulation with PWM proliferate and differentiate to IgM- and IgG-producing cells. The PWM-induced Ig production in B cells was dependent on T cells, and cell-free supernatant (CFS) obtained from PWM-stimulated PBL or T cell-rich fraction replaced T cell helper functions. The active substance(s) in CFS were most likely derived from T cells. The kinetic studies showed that the proliferation of B cells took place in advance of the final differentiation to Ig-producing cells and that T cells or T cell product(s) had to exist at the initiation of cultures in order to give the maximum helper effect. However, the final differentiation of B cells to Ig-producing cells was not dependent on T cells. The helper effect of T cells or T cell product(s) on PWM-induced proliferation and differentiation of B cells was exerted across the MHC barrier. This may make it possible to apply this experimental system to the assessment of quantitative and/or qualitative changes in human helper T cells in several immunologic diseases.