Publication | Open Access
Hepatitis B Core Antigen
30
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0
References
1975
Year
ImmunohematologyComplement FixationImmunocytochemical TechniqueImmunologyHepatitis BConcentrated Dane ParticlesViral HepatitisVaccine TargetImmunochemistryAntibody EngineeringLaboratory MedicineAllergyAutoimmunityAntibody ScreeningVaccinationHepatologyHepatitisAbstract Radioactive CoresMedicine
Abstract Radioactive cores were prepared from concentrated Dane particles by DNA polymerase reaction followed by CsCl density gradient centrifugation. Two radioactive peaks were obtained: one peak with an average density of 1.36 g/cm3 in CsCl contained cores that possessed full serologic reactivity; the other peak, with a density of 1.28 to 1.32 g/cm3, contained cores associated with globulin. A double antibody immunoprecipitation test was developed, using the radioactive heavy cores as a source of antigen. The test was at least 300 times as sensitive as complement fixation for detecting antibody to core.