Studies on the Inhibition of C56-Induced Lysis (Reactive Lysis)

Abstract

Abstract Multiple polyanions and polycations were tested for their ability to influence formation of from C, C7, and sheep erythrocytes. Six of 11 polyanions tested, including polyanethol sulfonate, heparin, and dextran sulfate, inhibited this reaction. By contrast, polycations (five of seven tested), including polybrene, protamine, and polyornithine, potentiated formation of EC. The inhibition was similar to that previously described for anionic serum factors termed C-INH, while the potentiation seemed to involve neutralization of serum C-INH. Thus, this step of the complement attack mechanism seems amenable to modulation by certain polyelectrolytes, and may thereby be susceptible to pharmacologic manipulation.