Overexpression of GmPHR1 Promotes Soybean Yield through Global Regulation of Nutrient Acquisition and Root Development

Abstract

MYB-CC transcription factors (TFs) are essential for plant growth and development. Members of the MYB-CC subfamily with long N terminal domains, such as phosphate starvation response 1 (PHR1) or PHR1-like TFs, have well documented functions, while those with short N terminal domains remain less understood. In this study, we identified a nodule specific MYB-CC transcription factor 1 (<i>GmPHR1</i>) in soybean that is different from other canonical PHR family genes in that <i>GmPHR1</i> harbors a short N terminal ahead of its MYB-CC domain and was highly induced by rhizobium infection. The overexpression of <i>GmPHR1</i> dramatically increased the ratio of deformed root hairs, enhanced subsequent soybean nodulation, and promoted soybean growth in pot experiments. The growth promotion effects of <i>GmPHR1</i> overexpression were further demonstrated in field trails in which two <i>GmPHR1-OE</i> lines yielded 10.78% and 8.19% more than the wild type line. Transcriptome analysis suggested that <i>GmPHR1</i> overexpression led to global reprogramming, with 749 genes upregulated and 279 genes downregulated, especially for genes involved in MYB transcription factor activities, root growth, and nutrient acquisition. Taken together, we conclude that <i>GmPHR1</i> is a key gene involved in the global regulation of nodulation, root growth, and nutrient acquisition in soybeans, and is thus a promising candidate gene to target for soybean yield enhancement.