Covalent organic framework-loaded silver nanoparticles as robust mimetic oxidase for highly sensitive and selective colorimetric detection of mercury in blood

Abstract

Covalent organic frameworks (COFs) were fabricated with a hierarchical flower-like hollow structure, possessing a large specific surface area, high porosity, and excellent environmental stability. <i>In situ</i> growth of noble silver nanoparticles (AgNPs) onto COFs was conducted yielding COF-Ag nanozymes. The structural advantages of COFs can ensure the uniform dispersion and effective size control of AgNPs. More interestingly, the oxidase-like catalytic activity of the obtained COF-Ag nanozymes could be enhanced in the presence of Hg²⁺ ions, which could specifically interact with AgNPs to form Ag-Hg alloys. A COF-Ag catalysis-based colorimetric platform was thereby constructed for highly selective and sensitive analysis of Hg²⁺ ions, showing a linear concentration range from 0.050 to 10.0 μM, with a limit of detection of about 3.7 nM. Besides, the developed colorimetric strategy was successfully applied for detecting Hg²⁺ ions in human blood with favorable detection recoveries, indicating its potential for applications in the biomedical analysis, environmental monitoring, and food safety fields.