Ocular Immunology and Inflammation · 2022 · 11 citations · 59 references
MedicineImmunologyOphthalmologyInflammationInnate ImmunityOcular ImmunologyImmunopathology Of The EyeNeuroinflammationOcular InflammationRetinal ImmunologyRetinal PathologyRetinal Vascular DiseaseFluorescein Angiography FindingsFundus Fluorescein AngiographyMonocyte-derived MacrophagesNeovascularizationPro-inflammatory Cytokine ExpressionRetinal MicrogliaRetinal NeuroinflammationRetinal Vascular Inflammation
This study aims to investigate the effect of a systemic lipopolysaccharide (LPS) stimulus in the course of laser-induced choroidal neovascularization (CNV) in C57BL/6 J mice. A group of CNV-subjected mice received 1 mg/kg LPS via the tail vein immediately after CNV induction. Mouse eyes were monitored in vivo with fluorescein angiography for 2 weeks. In situ hybridization and flow cytometry were performed in the retina at different time points. LPS led to increased fluorescein leakage 3 days after CNV, correlated with a large influx of monocyte-derived macrophages and increase of pro-inflammatory microglia/macrophages in the retina. Additionally, LPS enhanced Vegfα mRNA expression by Glul-expressing cells but not Aif1 positive microglia/macrophages in the laser lesion. These findings suggest that systemic LPS exposure has transient detrimental effects in the course of CNV through activation of microglia/macrophages to a pro-inflammatory phenotype and supports the important role of these cells in the CNV course.
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Eric A. Pierce, Robert L. Avery, E D Foley et al. · Proceedings of the National Academy of Sciences · 1995 · 1K citations · Full text