Long-term water use efficiency and non-structural carbohydrates of dominant tree species in response to nitrogen and water additions in a warm temperate forest

Abstract

Nitrogen (N) deposition tends to accompany precipitation in temperate forests, and vegetation productivity is mostly controlled by water and N availability. Many studies showed that tree species response to precipitation or N deposition alone influences, while the N deposition and precipitation interactive effects on the traits of tree physiology, especially in non-structural carbohydrates (NSCs) and long-term water use efficiency (WUE), are still unclear. In this study, we measured carbon stable isotope (δ¹³C), total soluble sugar and starch content, total phenols, and other physiological traits (<i>e</i>.<i>g</i>., leaf C:N:P stoichiometry, lignin, and cellulose content) of two dominant tree species (<i>Quercus variabilis</i> Blume and <i>Liquidambar formosana</i> Hance) under canopy-simulated N deposition and precipitation addition to analyze the changes of long-term WUE and NSC contents and to explain the response strategies of dominant trees to abiotic environmental changes. This study showed that N deposition decreased the root NSC concentrations of <i>L. formosana</i> and the leaf lignin content of <i>Q. variabilis</i>. The increased precipitation showed a negative effect on specific leaf area (SLA) and a positive effect on leaf WUE of <i>Q. variabilis</i>, while it increased the leaf C and N content and decreased the leaf cellulose content of <i>L. formosana</i>. The nitrogen-water interaction reduced the leaf lignin and total phenol content of <i>Q. variabilis</i> and decreased the leaf total phenol content of <i>L. formosana</i>, but it increased the leaf C and N content of <i>L. formosana</i>. Moreover, the response of <i>L. formosana</i> to the nitrogen-water interaction was greater than that of <i>Q. variabilis</i>, highlighting the differences between the two dominant tree species. The results showed that N deposition and precipitation obviously affected the tree growth strategies by affecting the NSC contents and long-term WUE. Canopy-simulated N deposition and precipitation provide a new insight into the effect of the nitrogen-water interaction on tree growth traits in a temperate forest ecosystem, enabling a better prediction of the response of dominant tree species to global change.