Zinc ion detection using a benzothiazole-based highly selective fluorescence “turn-on” chemosensor and its real-time application

Abstract

A new photochromic fluorescence chemosensor was devised and effectively synthesized using benzothiazole and imidazopyridine derivatives. A "turn-on" fluorescence sensor BIPP for Zn²⁺ detection was developed and has a quick response, excellent sensitivity, and remarkable selectivity over other metal ions. When Zn²⁺ was added to the BIPP solution, a new strong fluorescence emission peak at 542 nm formed with a considerable increase in intensity. The fluorescence color of the BIPP solution changed from blue to bright green. The binding ratio 8 : 2 was found between BIPP and Zn²⁺ by the results of Job's plot, HRMS and ¹H-NMR. The detection limit (LOD) of BIPP towards Zn²⁺ was determined to be 2.36 × 10^-8, which is remarkably low. The ability to detect Zn²⁺ in real water samples demonstrates that BIPP may also be used in environmental systems. Additionally, BIPP can be used to measure Zn²⁺ levels in living cells.