Comparison of <i>in-situ</i> versus <i>ex-situ</i> delivery of polyethylenimine-BMP-2 polyplexes for rat calvarial defect repair via intraoperative bioprinting

Abstract

Gene therapeutic applications combined with bio- and nano-materials have been used to address current shortcomings in bone tissue engineering due to their feasibility, safety and potential capability for clinical translation. Delivery of non-viral vectors can be altered using gene-activated matrices to improve their efficacy to repair bone defects.<i>Ex-situ</i>and<i>in-situ</i>delivery strategies are the most used methods for bone therapy, which have never been directly compared for their potency to repair critical-sized bone defects. In this regard, we first time explore the delivery of polyethylenimine (PEI) complexed plasmid DNA encoding bone morphogenetic protein-2 (PEI-pBMP-2) using the two delivery strategies,<i>ex-situ</i>and<i>in-situ</i>delivery. To realize these gene delivery strategies, we employed intraoperative bioprinting (IOB), enabling us to 3D bioprint bone tissue constructs directly into defect sites in a surgical setting. Here, we demonstrated IOB of an osteogenic bioink loaded with PEI-pBMP-2 for the<i>in-situ</i>delivery approach, and PEI-pBMP-2 transfected rat bone marrow mesenchymal stem cells laden bioink for the<i>ex-situ</i>delivery approach as alternative delivery strategies. We found that<i>in-situ</i>delivery of PEI-pBMP-2 significantly improved bone tissue formation compared to<i>ex-situ</i>delivery. Despite debates amongst individual advantages and disadvantages of<i>ex-situ</i>and<i>in-situ</i>delivery strategies, our results ruled in favor of the<i>in-situ</i>delivery strategy, which could be desirable to use for future clinical applications.