Advancing ⁸⁹Zr-immuno-PET in neuroscience with a bispecific anti-amyloid-beta monoclonal antibody - The choice of chelator is essential

Abstract

The accelerated approval of the monoclonal antibody (mAb) aducanumab as a treatment option for Alzheimer's Disease and the continued discussions about its efficacy have shown that a better understanding of immunotherapy for the treatment of neurodegenerative diseases is needed. ⁸⁹Zr-immuno-PET could be a suitable tool to open new avenues for the diagnosis of CNS disorders, monitoring disease progression, and assessment of novel therapeutics. Herein, three different ⁸⁹Zr-labeling strategies and direct radioiodination with ¹²⁵I of a bispecific anti-amyloid-beta aducanumab derivate, consisting of aducanumab with a C-terminal fused anti-transferrin receptor binding single chain Fab fragment derived from 8D3 (Adu-8D3), were compared <i>ex vivo</i> and <i>in vivo</i> with regard to brain uptake and target engagement in an APP/PS1 Alzheimer's disease mouse model and wild type animals. <b>Methods:</b> Adu-8D3 and a negative control antibody, based on the HIV specific B12 antibody also carrying C-terminal fused 8D3 scFab (B12-8D3), were each conjugated with NCS-DFO, NCS-DFO*, or TFP-<i>N</i>-suc-DFO-Fe-ester, followed by radiolabeling with ⁸⁹Zr. ¹²⁵I was used as a substitute for ¹²⁴I for labeling of both antibodies. 30 µg of radiolabeled mAb, corresponding to approximately 6 MBq ⁸⁹Zr or 2.5 MBq ¹²⁵I, were injected per mouse. PET imaging was performed 1, 3 and 7 days post injection (p.i.). All mice were sacrificed on day 7 p.i. and subjected to <i>ex vivo</i> biodistribution and brain autoradiography. Immunostaining on brain tissue was performed after autoradiography for further validation. <b>Results:</b> <i>Ex vivo</i> biodistribution revealed that the brain uptake of [⁸⁹Zr]Zr-DFO*-NCS-Adu-8D3 (2.19 ±0.12 %ID/g) was as high as for its ¹²⁵I-analog (2.21 ±0.15 %ID/g). [⁸⁹Zr]Zr-DFO-NCS-Adu-8D3 and [⁸⁹Zr]Zr-DFO-<i>N</i>-suc-Adu-8D3 showed significantly lower uptake (< 0.65 %ID/g), being in the same range as for the ⁸⁹Zr-labeled controls (B12-8D3). Autoradiography of [⁸⁹Zr]Zr-DFO*-NCS-Adu-8D3 and [¹²⁵I]I-Adu-8D3 showed an amyloid-beta related granular uptake pattern of radioactivity. In contrast, the [⁸⁹Zr]Zr-DFO-conjugates and the control antibody groups did not show any amyloid-beta related uptake pattern, indicating that DFO is inferior for ⁸⁹Zr-immuno-PET imaging of the brain in comparison to DFO* for Adu-8D3. This was confirmed by day 7 PET images showing only amyloid-beta related brain uptake for [⁸⁹Zr]Zr-DFO*-NCS-Adu-8D3. In wild type animals, such an uptake was not observed. Immunostaining showed a co-localization of all administered Adu-8D3 conjugates with amyloid-beta plaques. <b>Conclusion:</b> We successfully demonstrated that ⁸⁹Zr-immuno-PET is suitable for imaging and quantifying amyloid-beta specific brain uptake using a bispecific aducanumab brain shuttling antibody, Adu-8D3, but only when using the novel chelator DFO*, and not DFO, for labeling with ⁸⁹Zr.