Detachment of bovine corneal endothelial cell sheets by cooling-induced surface hydration of poly[(<i>R</i>)-3-hydroxybutyrate]-based thermoresponsive copolymer coating

Abstract

a cooling treatment at 4 °C for 20 min, and the viability of the detached cell sheet was found to be unaffected by the cooling. This work may potentially inspire more studies involving the non-covalent thermoresponsive polymer coatings for corneal tissue engineering applications.