Novel Hydrogel System Eliminates Subculturing and Improves Retention of Nonsenescent Mesenchymal Stem Cell Populations

Abstract

<b>Aim:</b> To compare the physiological behavior of mesenchymal stem/stromal cells (MSCs) within an expandable tissue-mimetic 3D system relative to <i>in vitro</i> expansion in a traditional 2D system. <b>Methods:</b> Adipose-derived MSCs (ASCs) were continuously cultured for 6 weeks on either 2D culture plastic or in a 3D hydrogel system that eliminated subculturing. ASCs were assessed for senescence, 'stem-like' MSC markers, and ability for their secretome to augment a secondary cell population. <b>Results:</b> The 3D hydrogel system resulted in an enhanced retention of more regenerative, nonsenescent ASC populations that exhibited increased expression of 'stem-like' MSC surface markers. <b>Conclusion:</b> This study introduces a proof-of-concept design for a novel modular 3D system that can improve <i>in vitro</i> expansion of stem-like cell populations for future regenerative therapies.