Breath Analysis as a Technique in Clinical Chemistry

Abstract

Abstract Breath possesses unique advantages as a specimen for clinical chemical analyses, including the continuous equilibrium of gases and volatile substances between expired alveolar air and the pulmonary blood circulation. Substances amenable to analysis in breath include O2, CO2, CO, and other gases, volatile organic compounds, and many drugs with sufficiently high vapor pressures at physiological temperatures. Practical aspects of breath sampling and breath analysis are discussed, exemplified by breath-alcohol analysis. The requirements for obtaining breath samples in equilibrium with the pulmonary blood circulation are delineated, and experimental data are presented for the significant breath-sample characteristics bearing on design of breath collection and storage systems (end-expiratory temperature, breath volumes).