The Decompensated Detrusor II: Evidence for Loss of Sarcoplasmic Reticulum Function After Bladder Outlet Obstruction in the Rabbit

Abstract

No AccessJournal of UrologyBladder Research1 Aug 1996The Decompensated Detrusor II: Evidence for Loss of Sarcoplasmic Reticulum Function After Bladder Outlet Obstruction in the Rabbit Stephen A. Zderic, Dorothea Rohrmann, Chaoliang Gong, Howard McC. Synder, John W. Duckett, Alan J. Wein, and Robert M. Levin Stephen A. ZdericStephen A. Zderic More articles by this author , Dorothea RohrmannDorothea Rohrmann More articles by this author , Chaoliang GongChaoliang Gong More articles by this author , Howard McC. SynderHoward McC. Synder More articles by this author , John W. DuckettJohn W. Duckett More articles by this author , Alan J. WeinAlan J. Wein More articles by this author , and Robert M. LevinRobert M. Levin More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65758-6AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: While it may lack the classic morphological pattern in striated muscle systems, there is ample evidence that smooth muscle also contains sarcoplasmic reticulum. These intracellular storage sites release calcium into the cytosol to generate contractile force in response to various stimuli. A major component of the sarcoplasmic reticulum is an adenosine triphosphate dependent ion pump, which serves to drive free calcium out of the cytosol back into this intracellular reservoir. This ion pump serves to maintain the intracellular calcium storage sites, and also as a marker of the sarcoplasmic reticulum. Materials and Methods: Muscle strip studies were performed to stratify the data into 3 major groups (controls, and compensated and decompensated obstructions) based on physiological performance. These were correlated with biochemical and molecular determinations of sarcoplasmic endoplasmic reticulum calcium, magnesium-adenosinetriphosphatase expression. Results: Our results demonstrate a remarkable loss of sarcoplasmic endoplasmic reticulum calcium-adenosinetriphosphatase activity in the decompensated group and a moderate loss in the compensated group. Conclusions: These data provide molecular support for our previous physiological studies in which we demonstrated an important role for intracellular calcium storage and release with normal bladder smooth muscle function. These data strongly support our contention that contractile dysfunction in bladder smooth muscle following outlet obstruction is partially mediated by changes in the mechanisms of intracellular calcium homeostasis. 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Google Scholar From the Divisions of Urology, Children's Hospital of Philadelphia, Hospital of the University of Pennsylvania and University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania.© 1996 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byClement M, Delaney D, Austin J, Sliwoski J, Hii G, Canning D, DiSanto M, Chacko S and Zderic S (2018) Activation of the Calcineurin Pathway is Associated With Detrusor Decompensation: A Potential Therapeutic TargetJournal of Urology, VOL. 176, NO. 3, (1225-1229), Online publication date: 1-Sep-2006.LEVIN R, HASS M, BELLAMY F, HORAN P, WHITBECK K, CHOW P, KUNG L and GOSLING J (2018) Effect of Oral Tadenan Treatment on Rabbit Bladder Structure and Function After Partial Outlet ObstructionJournal of Urology, VOL. 167, NO. 5, (2253-2259), Online publication date: 1-May-2002.STEIN R, HUTCHESON J, KRASNOPOLSKY L, CANNING D, CARR M and ZDERIC S (2018) THE DECOMPENSATED DETRUSOR V: MOLECULAR CORRELATES OF BLADDER FUNCTION AFTER REVERSAL OF EXPERIMENTAL OUTLET OBSTRUCTIONJournal of Urology, VOL. 166, NO. 2, (651-657), Online publication date: 1-Aug-2001.BRATSLAVSKY G, KOGAN B and LEVIN R (2018) URETHRA IS MORE SENSITIVE TO ISCHEMIA THAN BLADDER: EVIDENCE FROM AN IN VITRO RAT STUDYJournal of Urology, VOL. 165, NO. 6 Part 1, (2086-2090), Online publication date: 1-Jun-2001.STEIN R, HUTCHESON J, GONG C, CANNING D, CARR M and ZDERIC S (2018) THE DECOMPENSATED DETRUSOR IV: EXPERIMENTAL BLADDER OUTLET OBSTRUCTION AND ITS FUNCTIONAL CORRELATION TO THE EXPRESSION OF THE RYANODINE AND VOLTAGE OPERATED CALCIUM CHANNELSJournal of Urology, VOL. 165, NO. 6 Part 2, (2284-2288), Online publication date: 1-Jun-2001.BURKHARD F, LEMACK G, ZIMMERN P, LIN V and Mc CONNELL J (2018) CONTRACTILE PROTEIN EXPRESSION IN BLADDER SMOOTH MUSCLE IS A MARKER OF PHENOTYPIC MODULATION AFTER OUTLET OBSTRUCTION IN THE RABBIT MODELJournal of Urology, VOL. 165, NO. 3, (963-967), Online publication date: 1-Mar-2001.STEIN R, GONG C, HUTCHESON J, CANNING D and ZDERIC S (2018) THE DECOMPENSATED DETRUSOR III: IMPACT OF BLADDER OUTLET OBSTRUCTION ON SARCOPLASMIC ENDOPLASMIC RETICULUM PROTEIN AND GENE EXPRESSIONJournal of Urology, VOL. 164, NO. 3 Part 2, (1026-1030), Online publication date: 1-Sep-2000.GOSLING J, KUNG L, DIXON J, HORAN P, WHITBECK C and LEVIN R (2018) CORRELATION BETWEEN THE STRUCTURE AND FUNCTION OF THE RABBIT URINARY BLADDER FOLLOWING PARTIAL OUTLET OBSTRUCTIONJournal of Urology, VOL. 163, NO. 4, (1349-1356), Online publication date: 1-Apr-2000.AZADZOI K, TARCAN T, KOZLOWSKI R, KRANE R and SIROKY M (2018) OVERACTIVITY AND STRUCTURAL CHANGES IN THE CHRONICALLY ISCHEMIC BLADDERJournal of Urology, VOL. 162, NO. 5, (1768-1778), Online publication date: 1-Nov-1999.LEMACK G, BURKHARD F, ZIMMERN P, MCCONNELL J and LIN V (2018) PHYSIOLOGIC SEQUELAE OF PARTIAL INFRAVESICAL OBSTRUCTION IN THE MOUSE: ROLE OF INDUCIBLE NITRIC OXIDE SYNTHASEJournal of Urology, VOL. 161, NO. 3, (1015-1022), Online publication date: 1-Mar-1999. Volume 156Issue 2SAugust 1996Page: 587-592 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.MetricsAuthor Information Stephen A. Zderic More articles by this author Dorothea Rohrmann More articles by this author Chaoliang Gong More articles by this author Howard McC. Synder More articles by this author John W. Duckett More articles by this author Alan J. Wein More articles by this author Robert M. Levin More articles by this author Expand All Advertisement PDF downloadLoading ...