Autocrine/Paracrine Activation of the GABA_AReceptor Inhibits the Proliferation of Neurogenic Polysialylated Neural Cell Adhesion Molecule-Positive (PSA-NCAM⁺) Precursor Cells from Postnatal Striatum

Abstract

GABA and its type A receptor (GABA A R) are present in the immature CNS and may function as growth-regulatory signals during the development of embryonic neural precursor cells. In the present study, on the basis of their isopycnic properties in a buoyant density gradient, we developed an isolation procedure that allowed us to purify proliferative neural precursor cells from early postnatal rat striatum, which expressed the polysialylated form of the neural cell adhesion molecule (PSA-NCAM). These postnatal striatal PSA-NCAM cells were shown to proliferate in the presence of epidermal growth factor (EGF) and formed spheres that preferentially generated neurons in vitro. We demonstrated that PSA-NCAM neuronal precursors from postnatal striatum expressed GABA A R subunits in vitro and in situ. GABA elicited chloride currents in PSA-NCAM cells by activation of functional GABA A R that displayed a typical pharmacological profile. GABA A R activation in PSA-NCAM cells triggered a complex intracellular signaling combining a tonic inhibition of the mitogen-activated protein kinase cascade and an increase of intracellular calcium concentration by opening of voltagegated calcium channels. We observed that the activation of GABA A R in PSA-NCAM neuronal precursors from postnatal striatum inhibited cell cycle progression both in neurospheres and in organotypic slices. Furthermore, postnatal PSA-NCAM striatal cells synthesized and released GABA, thus creating an autocrine/paracrine mechanism that controls their proliferation. We showed that EGF modulated this autocrine/paracrine loop by decreasing GABA production in PSA-NCAM cells. This demonstration of GABA synthesis and GABA A R function in striatal PSA-NCAM cells may shed new light on the understanding of key extrinsic cues that regulate the developmental potential of postnatal neuronal precursors in the CNS.