Analytical Chemistry · 2022 · 55 citations · 42 references
Intracellular lipid storage and regulation occur in lipid droplets, which are of great significance to the physiological activities of cells. Herein, a lipid droplet-specific fluorescence probe (<b>lip-YB</b>) with a high quantum yield (QY<sub><b>lip-YB</b></sub> = 73.28%), excellent photostability, and quickly polarity sensitivity was constructed successfully. Interestingly, <b>lip-YB</b> exhibited remarkable two-photon (TP) characteristics, which first realized real-time monitoring of the lipid droplet multidynamics process, diagnosing nonalcoholic fatty liver disease (NAFLD) and inflammation in living mice via TP fluorescence imaging. It is found that the as-prepared <b>lip-YB</b> provides a new avenue to design lipid droplet-specific imaging probes, clarifies its roles and mechanisms in cell metabolism, and can timely intervene in lipid droplet-related diseases during various physiological and pathological processes.
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