Short- and Long-Term Exposure to Heat Stress Differently Affect Performance, Blood Parameters, and Integrity of Intestinal Epithelia of Growing Pigs

Abstract

The effect of short- and long-term exposure to heat stress (HS) was analyzed on blood components, performance, and intestinal epithelium integrity of pigs. Eighteen pigs (36.0 ± 3.5 kg BW) were assigned to three groups: thermo-neutral (TN); 2 d exposure to HS (2dHS); and 7 d exposure to HS (7dHS). Blood chemistry and hemogram analyses were performed; small intestine samples were analyzed for mRNA expression and histology. Compared to TN, 2dHS and 7dHS pigs reduced weight gain and feed intake; weight gain was higher in 7dHS than in 2dHS pigs (p < 0.05). White blood cells, platelet, and hematocrit were affected in 2dHS and 7dHS compared to TN pigs (p < 0.05). Short- and long-term exposure to HS affected blood concentration of triglycerides, urea, total protein, and albumin (p ≤ 0.05). Villi-height and crypt-depth decreased in HS pigs (p < 0.01). Mucin-producing and apoptotic cell number increased in 7dHS compared to TN pigs (p < 0.05). Expression of tight-junction-proteins decreased in 2dHS pigs compared to TN and 7dHS pigs (p < 0.05). Short-term exposure of pigs to HS dramatically affects performance, blood components, and integrity of the small intestine epithelia; nevertheless, pigs show signs of recovery at 7 d of HS exposure.