Veterinary Sciences · 2022 · 11 citations · 31 references
Veterinary MedicineInfectious DiseasesMolecular BiologyMolecular DiagnosticsMolecular VirologyVirologyPathogen DetectionPoultry Infectious DiseasesPoultry VirologyVeterinary VirologyViral DiagnosticsViral Diseases Of PoultryDiagnostic Virology In PoultryHemorrhagic Viral DiseaseInfectious Disease DiagnosisLower Limit Of QuantificationMolecular Detection Of PathogensPolymerase Chain Reaction SpecificityViral Disease DiagnosticsVirus Detection Assays
Gosling plague (GP) is an acute and hemorrhagic infectious disease caused by goose parvovirus (GPV). The goose industry suffers significant economic losses as a result of GP, which is found to be widespread worldwide, with high rates of morbidity and mortality. Our group developed a novel technique for detecting GPV nanoparticle-assisted polymerase chain reaction (nanoPCR) and the characterization of its specificity and sensitivity. It was developed by using the traditional polymerase chain reaction (PCR) and nanoparticles. The findings of this study revealed that GPV nanoPCR products were 389 bp in length, and the lower limit of the nanoPCR assay was 4.68 × 102 copies/μL, whereas that of the conventional PCR assay was 4.68 × 104 copies/μL. A total of 230 geese suspected of GPV were detected using nanoPCR, with a positive rate of 83.0% and a specificity of 73%, respectively. Overall, we present a hitherto undocumented method for identifying GPV by using nanoPCR to aid in the evaluation of subclinical illness.
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Jianke Wang, Yuening Cheng, Miao Zhang et al. · BMC Veterinary Research · 2015 · 153 citations · Full text
Gold nanoparticles for high-throughput genotyping of long-range haplotypes
Peng Chen, Dun Pan, Chunhai Fan et al. · Nature Nanotechnology · 2011 · 113 citations