Electrochemical Immunosensor for Detection of <i>H. pylori</i> Secretory Protein VacA on g-C₃N₄/ZnO Nanocomposite-Modified Au Electrode

Abstract

A g-C₃N₄/ZnO (graphitic carbon nitride/zinc oxide) nanocomposite-decorated gold electrode was employed to design an antigen-antibody-based electrochemical biosensor to detect <i>Helicobacter pylori</i> specific toxin, vacuolating cytotoxin A (VacA). The thermal condensation method was used to synthesize the g-C₃N₄/ZnO nanocomposite, and the nanocomposite was deposited electrochemically on a gold electrode. The morphology as well as the structure of the synthesized nanocomposite were confirmed by scanning electron microscopy, energy-dispersive X-ray analysis, X-ray diffraction, and Fourier transform infrared techniques. The nanocomposite efficiently increased the sensor performance by amplifying the signals. EDC-NHS chemistry was exploited for attachment of VacA antibodies covalently with the g-C₃N₄/ZnO-modified gold electrode. This modified electrode was exploited for immunosensing of <i>H. pylori</i>-specific VacA antigen. The immunosensor was stable for up to 30 days and exhibited good sensitivity of 0.3 μA^-1 ng mL^-1 in a linear detection range of 0.1 to 12.8 ng mL^-1. Apart from this, the fabricated sensor showed unprecedented reproducibility and remarkable selectivity toward the <i>H. pylori</i> toxin VacA. Thus, the highly sensitive immunosensor is a desirable platform for <i>H. pylori</i> detection in practical applications and clinical diagnosis.