The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

Abstract

Fatty acid synthase (FAS) is a key enzyme in the lipid synthesis pathway, however, its roles in insects remain largely unknown. Here, we firstly identified two <i>FAS</i> genes from the transcriptome dataset of the general cutworm <i>Spodoptera litura</i>, which is a destructive insect pest of many crops. Both <i>SlFAS1</i> and <i>SlFAS2</i> were highly expressed in third instar larvae and in their fat bodies. Then, we successfully silenced <i>SlFAS1</i> in third instar larvae and the content of α-linolenic acid and triglyceride was significantly decreased. Besides that, the effect of FAS on the metamorphic development in <i>S. litura</i> was evaluated. The results indicate that after silencing <i>SlFAS1</i>, the survival rates of <i>S. litura</i> larvae decreased significantly compared to the control groups. Silencing <i>SlFAS1</i> in fifth instar larvae resulted in more malformed pupae and adults, and the emergence rates were significantly reduced. Furthermore, the ecdysone content in the haemolymph of fifth instar larvae was significantly decreased after silencing <i>SlFAS1</i>. In addition, knocking down <i>SlFAS1</i> significantly alters the expression of other key genes in the lipogenesis pathway, implying that FAS has an impact on the lipogenesis pathway. The present study deepens the understanding of FAS in insects and provides novel potential targets for managing insect pests.