Publication | Open Access
Nitrate Reduction in Leaves of Grapevine and Other Fruit Trees1
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1978
Year
BotanyBiochemistryNitrate ReductionGrapevine LeavesPhysiologyNo 3Natural SciencesEnzyme ActivityPlant PathologyPlant MetabolismPost-harvest PhysiologyMetabolismMedicinePhytotoxicityPlant PhysiologyHorticultural ScienceOxidative Stress
Abstract Optimum conditions for in vivo activity of nitrate reductase (NR) from grape leaves ( Vitis vinifera L.) were pH 7.5, 100 m m NO 3 substrate, and incubation at 40°C. Pretreatment with low concentrations of NO 3 (0.05 m ) at room temperature did not increase activity substantially. Induction of NR by NO 3 substrate was investigated. Enzyme activity was maximum at the highest NO 3 levels. Intact-tissue assay detected NR in mature leaves of grapevines, walnut ( Junglans regia L.), plum ( Prunus domestica L.), pears ( Pyrus communis L.), and sweet cherry ( Prunus avium L.) grown under field conditions. In addition, a particulate NR was demonstrated in walnuts and grapevine leaves by in vitro assay.