Nano-Iron and Nano-Zinc Induced Growth and Metabolic Changes in Vigna radiata

Abstract

The widespread industrial use and consequent release of nanosized iron (nFe3O4) and zinc oxide (nZnO) particles into the environment have raised concerns over their effects on living organisms, including plants. These nanoparticles are the source of their respective metal ions and although plants require both Fe and Zn ions for proper growth, excessive levels of these metals are toxic to them. A better understanding of the effects of these nanoparticles on plants also offers an opportunity for their useful applications in agriculture. The present work evaluates the changes in seed germination, plant growth, photosynthetic capacity, levels of biomolecules and antioxidant enzymes in Vigna radiata (L.) Wilczek when grown in the presence of nFe3O4 (size 1–4 nm) and nZnO (size 10–20 nm) and compared to the control plants. The plants were raised hydroponically for up to 14 days at two different concentrations of nanoparticles, viz. 10 and 100 mg/L. Inductively coupled plasma mass spectrometry (ICP-MS) results established that V. radiata can accumulate Fe and Zn in shoots with high efficiency. The results indicated that nFe3O4 had a favourable effect on V. radiata, whereas no apparent benefit or toxicity of nZnO was observed at the tested concentrations.