The Shift of Soil Bacterial Community After Afforestation Influence Soil Organic Carbon and Aggregate Stability in Karst Region

Abstract

Soil microbes regulate the carbon cycle and affect the formation and stabilization of soil aggregates. However, the interactions between the soil microbial community and soil organic carbon (SOC) fractions, organic carbon (OC) content in aggregates, and soil aggregate stability after afforestation are remain poorly understood. In our study, we investigated SOC fractions in bulk soil, aggregate-associated OC content, soil aggregate stability, and soil bacterial community with high-throughput 16S rRNA sequencing at sites representing natural secondary forest (NF) and managed forest (MF), with cropland (CL) as reference in a degraded karst region of Southwest China. Our results showed that afforestation remarkably increased the SOC fraction and OC content in aggregates, the mean weight diameter (MWD), and the mean geometric diameter (GMD). The most dominant bacterial phyla detected were <i>Acidobacteriota</i>, <i>Actinobacteriota</i>, <i>Proteobacteria</i>, and <i>Chloroflexi</i> across all soils. Afforestation remarkably altered the relative abundances of most of the dominant soil bacteria at the phylum, class, and order levels. Interestingly, such changes in the abundance of soil bacteria taxa had significantly effects on SOC fraction, aggregate-associated OC content, MWD, and MGD. The abundance of dominant bacterial taxa such as <i>Methylomirabilota</i>, <i>Latescibacterota</i>, <i>Methylomirabilia</i>, <i>MB-A2-108</i>, <i>norank_Latescibacterota</i>; <i>Dehalococcoidia</i>, <i>Rokubacteriales</i>, <i>Gaiellales</i>, <i>Microtrichales</i>, <i>norank_c__MB-A2-108</i>, <i>norank_c__norank_p__Latescibacterota</i>, <i>Rhizobiales</i>, and <i>S085</i> not only remarkably increased but also had significant positive effects on SOC fractions and aggregate-associated OC content after afforestation. Moreover, MWD and MGD were positively correlated with the relative abundance of <i>Methylomirabilota</i>, <i>Methylomirabilia</i>, <i>Rokubacteriales</i>, <i>Latescibacterota</i>, and <i>Rhizobiales</i>. Results indicated the importance of certain soil bacteria for regulating SOC storage and soil aggregate stability. We concluded that afforestation on cropland could alter the abundance of soil bacteria, and these changes modulate the stability of soil aggregates and SOC fractions.