Immunomodulatory effect of Linalool (Lin) against CCl₄‐induced hepatotoxicity and oxidative damage in rats

Abstract

Abstract The current study explored the hepatoprotective and immunomodulatory effects of Linalool (Lin) against carbon tetrachloride (CCl 4 )‐induced toxicity in mice. Four study groups ( n = 8 each) were used: (1) a negative control group and (2) a toxicity control group (single dose of CCl 4 administered on day 14 as 1 mL/kg of CCL 4 in 1% olive oil). Intraperitoneally (i.p.)), and two experimental groups where mice were treated with either (3) Lin (25 mg/kg b.w., orally, daily for 15 days) or (4) pretreated with Lin (25 mg/kg b.w., orally, daily for 14 days) and intoxicated with CCl 4 (1 mL/kg of CCL 4 in 1% olive oil. i.p.) on day 14. The levels of the anti‐inflammatory cytokine interleukin 10 (IL‐10), the proinflammatory cytokines TNF‐α, IL‐6, and TGF‐1β, and the histopathology of the liver were assessed. According to our findings, IL‐10 concentrations were significantly increased in Lin‐treated groups, while other cytokine levels were marked by a considerable decrease in the toxicity model group (CCl 4 ‐treated group). Histopathological examinations of liver tissues showed that the Lin‐treated groups had an almost normal structure. The current findings showed that Lin could inhibit CCl 4 ‐induced liver injury in mice, which warrants further investigation of Lin as a potential protective and therapeutic agent against hepatotoxicity.