Ratiometric fluorescence enzyme-linked immunosorbent assay based on carbon dots@SiO₂@CdTe quantum dots with dual functionalities for alpha-fetoprotein

Abstract

@CdTe QDs changed from pink to purple to blue with the increasing AFP level when viewed by the naked eye. Good reproducibility, high specificity, and acceptable stability were achieved for the analysis of target AFP. Importantly, the accuracy of ratiometric fluorescence immunoassay was evaluated to determine human serum samples, giving well-matched results relative to commercially usable human AFP ELISA method.