In situ green synthesis of Cu-doped ZnO based polymers nanocomposite with studying antimicrobial, antioxidant and anti-inflammatory activities

Abstract

Abstract The use of eco-friendly methods for the synthesis of nanoparticles and its nano-composite has become a public demand nowadays to reduce the risks of chemical methods. In the current study, green synthesis of Cu-doped ZnO based polymers nan-ocomposite was performed. Various instrumental analysis including UV–vis, ATR-FTIR spectroscopy, XRD, SEM coupled with energy dispersive X-ray analysis, TEM and Thermal gravimetric were used to characterize nano-composite. Highly antibacterial activity of the synthesized nano-composite was recorded against tested microorganisms with promising efficacy against bacteria namely; Bacillus subtilis , Staphylococcus aureus , Enterococcus faecalis , Proteus vulgaris, Pseudomonas aeruginosa , Escherichia coli , Salmonella typhimurium and yeast ( Candida albicans ) but unfortunately not against black fungus ( Mucor circinelloides ) and filamentous fungi Aspergillus flavus and A. niger. Anti-inflammatory of nano-composite represented by hemolysis inhibition was observed at using low concentration (100 µg/mL) with enhancing 23.85% compared with free nano-composite while at high concentrations 500 and1000 µg/mL the anti-inflammatory activity was approximately similar with enhancing 3.91% and 1.99%, respectively. Antioxidant of the nano-composite was better than the antioxidant of free nano-composite at all tested concentrations, moreover the IC 50 of the nano-composite (91.16 µg/mL) was less than the IC 50 , (203.65 µg/mL) of the free nano-composite.