Genome-Wide Identification of MDH Family Genes and Their Association with Salt Tolerance in Rice

Abstract

Malate dehydrogenase (MDH) is widely present in nature and regulates plant growth and development, as well as playing essential roles, especially in abiotic stress responses. Nevertheless, there is no comprehensive knowledge to date on <i>MDH</i> family members in rice. In this study, a total of 12 <i>MDH</i> members in rice were identified through genome-wide analysis and divided into three groups on the basis of their phylogenetic relationship and protein-conserved motifs. Evolutionary analysis showed that MDH proteins from rice, maize and wheat shared a close phylogenetic relationship, and the <i>MDH</i> family was conserved in the long-term process of domestication. We identified two segmental duplication events involving four genes, which could be the major force driving the expansion of the <i>OsMDH</i> family. The expression profile, cis-regulatory elements and qRT-PCR results of these genes revealed that a few <i>OsMDH</i> showed high tissue specificity, almost all of which had stress response elements in the promoter region, and ten <i>MDH</i> members were significantly induced by salt stress. Through gene-based association analysis, we found a significant correlation between salt tolerance at the seedling stage and the genetic variation of <i>OsMDH8.1</i> and <i>OsMDH12.1</i>. Additionally, we found that the polymorphism in the promoter region of <i>OsMDH8.1</i> might be related to the salt tolerance of rice. This study aimed to provide valuable information on the functional study of the rice <i>MDH</i> gene family related to salt stress response and revealed that <i>OsMDH8.1</i> might be an important gene for the cultivar improvement of salt tolerance in rice.