Magnetic Nanoparticle-Reinforced Dual-Template Molecularly Imprinted Polymer for the Simultaneous Determination of Oxazepam and Diazepam Using an Electrochemical Approach

Abstract

Abstract In the current research, an electrochemical sensor was fabricated to simultaneously quantify oxazepam and diazepam based on reinforced magnetic multi-walled carbon nanotube molecularly imprinted polymer/pencil graphite electrode (MIP/MMWCNT/PGE). MIP modified with MWCNTs was prepared using methacrylic acid as a functional monomer and oxazepam and diazepam as templates. SEM images of obtained MIP nanocomposite showed the dimensions in the range of 26–36 nm. Central composite design was used to optimize the effective factors. Under the optimized conditions, the linear concentration ranges and the limits of detection were obtained as 0.01–200, 0.05–150 µM and 59, 21 nM for oxazepam and diazepam, respectively. The results show that the designed sensor bears good stability, repeatability, and reproducibility. Moreover, the interference effects of various compounds on the measurement of the above medicines were studied. The designed sensor may be used for the simultaneous measurement of oxazepam and diazepam in medicinal and biological samples. The results were validated and compared with results obtained by commonly used techniques available in the literature.