Radioenzymatic Assay for Direct Measurement of Plasma Pyridoxal 5'-Phosphate

Abstract

Abstract Vol. 29 p 642: In column two, sixth line from the bottom, change "25 000" to "54 000." Insert an addition (italicized here) to the next to last line in that column so that it reads".. ., then dialyzed overnight against the same buffer at 4 °C, then divided..." p 843: In line six under Results and Discussion, change "mg" to "µg" p 1537: In revision of this paper a paragraph of introduction was inadvertently omitted (which cites refs. 1-5), as were the first two paragraphs under Materials and Methods, which should read as follows: Human placentas were obtained from the obstetrical department within 12 h of delivery. Fresh human abdominal muscle was obtained within 12 h of autopsy. All tissues were stored at 4 °C and processed, as described below, within 6 h. Placenta and abdominal muscle were stripped free of membranes and blood vessels, cut into small pieces, and extensively washed at 4 °C with cold saline (NaCl, 9 g/L). Muscle CK-MM and placental CK-BB were purified according to the method of Armstrong et al. (6), with the following modifications: we used dithioerythritol (Sigma Chemical Co., St. Louis, MO 63178), 15 mmol/L, instead of 2-mercaptoethanol, omitted the final (NH4)2SO4 precipitation and final Sephadex G-100 fractionation, and used DE-52 (Whatman Ltd., Maidstone, Kent, U.K.) instead of DEAE-cellulose (Cellex-D; Bio-Rad Laboratories, Richmond, CA 94804). p 2026: First paragraph, lines nine and 16: delete "500" (the Dextran used in the cited reference was a 50 000-dalton preparation, not the 500 000-Da Pharmacia preparation). p 2029: In line 10 of the first full paragraph in column two, change "1.171" to "0.117." p 2125: First two columns and Table 1: All lipase results expressed as kU/L should be divided by 3600. Vol. 30 p 170: The next-to-last paragraph was inadvertently omitted in layout and should read as follows: If the sample was first incubated at 37 °C until the serum became clear (i.e., until the cryoprecipitate dissolved) and the enzyme activity immediately determined at 30 °C (Figure 2, curves 1 and 2), normal activity was observed.