Publication | Closed Access
Direct Alkylation of Amines with Primary and Secondary Alcohols through Biocatalytic Hydrogen Borrowing
33
Citations
36
References
2017
Year
Bioorganic ChemistryEngineeringOrganic ChemistryChemistryBiocatalytic Hydrogen BorrowingChemical EngineeringReductive AminaseDirect AlkylationBiochemical EngineeringNatural Product BiosynthesisHomogeneous CatalysisSecondary AlcoholsAlcohol DehydrogenasesAsp RedamSingle Alcohol DehydrogenaseBiochemistryBiocatalysisCatalysisHydrogenCatalytic SynthesisNatural SciencesEnzyme CatalysisBiotechnologyMolecular Catalysis
Abstract The reductive aminase from Aspergillus oryzae ( Asp RedAm) was combined with a single alcohol dehydrogenase (either metagenomic ADH‐150, an ADH from Sphingobium yanoikuyae (SyADH), or a variant of the ADH from Thermoanaerobacter ethanolicus ( Te SADH W110A)) in a redox‐neutral cascade for the biocatalytic alkylation of amines using primary and secondary alcohols. Aliphatic and aromatic secondary amines were obtained in up to 99 % conversion, as well as chiral amines directly from the racemic alcohol precursors in up to >97 % ee , releasing water as the only byproduct.
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