Isolation of Monoclonal Antibodies Specific for Human c-<i>myc</i> Proto-Oncogene Product

TLDR

Six monoclonal antibodies were isolated from mice immunized with synthetic peptides derived from the human c‑myc gene product. The study aims to develop reagents that enable an immunoblotting assay to quantify p62c‑myc in cells. The authors use these monoclonal antibodies to establish the immunoblotting assay for p62c‑myc. Five of the six antibodies precipitate p62c‑myc from human cells, three also recognize mouse c‑myc, none bind chicken p110gag‑myc, and all six detect p62c‑myc on immunoblots.

Abstract

Six monoclonal antibodies have been isolated from mice immunized with synthetic peptide immunogens whose sequences are derived from that of the human c-myc gene product. Five of these antibodies precipitate p62c-myc from human cells, and three of these five also recognize the mouse c-myc gene product. None of the antibodies sees the chicken p110gag-myc protein. All six antibodies recognize immunoblotted p62c-myc. These reagents also provide the basis for an immunoblotting assay by which to quantitate p62c-myc in cells.