Development of diabetes mellitus in aging transgenic mice following suppression of pancreatic homeoprotein IDX-1

Abstract

MethodsPlasmid and transgene construction.The pCMV-IDX-1 expression plasmid, encoding the cDNA for rat IDX-1; the -4.6-kbpIDX-1-pGL3 plasmid, encoding -4.6 kb of the mouse IDX-1 promoter and a luciferase reporter; and the pcDNA3-E12 and pcDNA3-E47 expression plasmids have been described previously (24-26).FarFlat-CAT, encoding pentamerized glucose-responsive elements Far and Flat derived from the rat insulin I promoter, was a gift from L.G. Moss