A Wireless Electrochemiluminescence Detector Applied to Direct and Indirect Detection for Electrophoresis on a Microfabricated Glass Device

Abstract

A novel electrochemiluminescence (ECL) detector is\npresented in this article. The detector is applied for\nmicellar electrokinetic chromatographic separation of\ndichlorotris(2,2‘-bipyridyl)ruthenium(II) hydrate [Ru(bpy)] and dichlorotris(1,10-phenanthroline)ruthenium(II) hydrate [Ru(phen)] on a microfabricated glass device.\nIt consists of a microfabricated “U”-shape floating platinum electrode placed across the separation channel. The\nlegs of the U function respectively as working and counter\nelectrode. The required potential difference for the ECL\nreaction is generated at the Pt electrode by the electric\nfield available in the separation channel during electrophoretic separation. Initial experiments demonstrate a\nmicellar electrokinetic separation and direct ECL detection of 10<sup>-16</sup> mol of Ru(phen) (10<sup>-6</sup> M) and 4.5 × 10<sup>-16</sup>\nmol of Ru(bpy) (5 × 10<sup>-6</sup> M). Also, preliminary results\nshow the indirect detection of three amino acids. The high\nvoltage at the location of detection does not interfere with\nthe electrochemistry.