Characterization of the denitrifying fraction of phosphate accumulating organisms in biological phosphate removal

Abstract

Research Article| January 01 1999 Characterization of the denitrifying fraction of phosphate accumulating organisms in biological phosphate removal Jens Meinhold; Jens Meinhold *Department of Chemical Engineering, Technical University of Denmark, DK-2800 Lyngby, Denmark Search for other works by this author on: This Site PubMed Google Scholar Carlos D. M. Filipe; Carlos D. M. Filipe **Environmental Systems Engineering, Clemson University, Clemson, SC 29634-0919, USA Search for other works by this author on: This Site PubMed Google Scholar Glen T. Daigger; Glen T. Daigger ***CH2M HILL, 100 Inverness Terrace East, Englewood, CO 80122, USA Search for other works by this author on: This Site PubMed Google Scholar Steven Isaacs Steven Isaacs †Krüger A/S, Gladsaxevej 363, DK-2860 Soeborg, Denmark Search for other works by this author on: This Site PubMed Google Scholar Water Sci Technol (1999) 39 (1): 31–42. https://doi.org/10.2166/wst.1999.0007 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Share Icon Share Twitter LinkedIn Tools Icon Tools Cite Icon Cite Permissions Search Site Search nav search search input Search input auto suggest search filter All ContentAll JournalsThis Journal Search Advanced Search Citation Jens Meinhold, Carlos D. M. Filipe, Glen T. Daigger, Steven Isaacs; Characterization of the denitrifying fraction of phosphate accumulating organisms in biological phosphate removal. Water Sci Technol 1 January 1999; 39 (1): 31–42. doi: https://doi.org/10.2166/wst.1999.0007 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex Results of experimental investigations are presented that strongly support the hypothesis that PAO from activated sludge systems consist of two groups: a) denitrifying PAO (DNPAO) capable of using oxygen and nitrate and b) non-denitrifying PAO (non-DNPAO) only able to use oxygen. Batch experiments were performed in which activated sludge obtained from a pilot scale BiodeniphoTM was submitted to a sequence of anaerobic/anoxic/aerobic, anaerobic/aerobic or anaerobic/anoxic conditions while monitoring the course of NOx-N, NH4-N, PO4-P, PHB and PHV. Several methods for the determination of the two fractions of PAO are performed and compared.This study extends on previously reported results (Kern-Jespersen and Henze, 1993) in that the pH was controlled to around pH 7 to assure that phosphate precipitation was minimal, and in the measurement of PHB and PHV. With regards to the latter, the paper also examines the influence of the size of the internal PHA pool on the anoxic phosphorus uptake rate. Simulations implementing existing models for the growth of non-DNPAO and DNPAO are used to confirm the experimental results and to gain a better understanding of some of the observations. Phosphorus removal, denitrification, DNPAO, non-DNPAO, activated sludge, simulation, saturation kinetics, PHB This content is only available as a PDF. © IWA Publishing 1999 You do not currently have access to this content.