Characterization of Membrane Proteins in Isolated Native Cellular Membranes by Dynamic Nuclear Polarization Solid‐State NMR Spectroscopy without Purification and Reconstitution

Abstract

Membranproteine in ihrer natürlichen Zellmembran können ohne vorherige Reinigung durch Kombination dynamischer Kernpolarisation mit Festkörper-NMR-Spektroskopie unter Probenrotation um den magischen Winkel untersucht werden (siehe Bild). Die Empfindlichkeit, die zum Beobachten der Membranproteine notwendige ist, wird durch dynamische Kernpolarisation erreicht. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.