Mechanistic Insight into Enzymatic Glycosyl Transfer with Retention of Configuration through Analysis of Glycomimetic Inhibitors

Abstract

Strukturelle „Valid“-ierung: Der Mechanismus des enzymkatalysierten konfigurationserhaltenden Glycosyltransfers gibt Rätsel auf, zumal aussagekräftige 3D-Strukturen ternärer Inhibitor-Enzym-Komplexe fehlen. Durch die Synthese und mehrdimensionale kinetische Analyse von Validoxylaminderivaten wurde die 3D-Struktur eines ternären Komplexes erhalten (siehe Bild; U=Uridyl), der Einblick in die Geometrie und das Donor-Akzeptor-Wechselspiel am Glycosyltransferzentrum gibt. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.