Factors That Influence Mobility, Resolution, and Selectivity in Capillary Zone Electrophoresis. III. The Role of the Buffers' Anion

Abstract

Abstract A study of the anion of the buffer and its effect on electroosmotic flow, mobility times, resolution and selectivity was undertaken. The anions selected acetate, borate, phosphate, citrate, carbonate, nitrate and nitrite, gave widely different currents at the same applied voltage (20 kV). Carbonate produced the lowest current, while citrate produced the highest current. Also, it was found that the anion used influences not only electroosmotic flow and mobility times but the resolution and selectivity of eight dansylated amino acids. It is hereby recommended that more attention be paid to the selection of a buffer.